Question 1

The use of which of the following would be most appropriate for inhibiting transcription of a specific gene?

Accepted Answer

A)   Antisense RNA
B)   Interference RNA
C)   DNA chips
D)   Homologous recombination
E)   PCR

Question 2

The process of inserting recombinant DNA is considered transfection when

Accepted Answer

A)   the host cells are yeast cells.
B)   the host cells are E. coli cells.
C)   the host cells are mouse tissue culture cells.
D)   the vector lacks a replicon.
E)   a selectable marker is used.

Question 3

Describe three useful products that have been produced using biotechnology. Outline two specific dangers that could result from producing organisms that contain foreign genes.

Accepted Answer

Useful products include rice grains that...

Question 4

All other things being equal, which of the following genetically altered foods would most likely be of concern regarding human health?

Accepted Answer

A)   Food in which the B. thuringiensis toxin has been added
B)   Food that contains an inserted gene that allows plants to grow better in high salt conditions
C)   Food that contains an inserted gene that allows plants to grow faster
D)   Food that contains an inserted gene that produces  β\betaβ -carotene in beans
E)   Both a and b

Question 5

Variation at a specific gene is known to affect metabolism in mice, but it is not clear which of the nucleotides are responsible for the changes. In addition to recombinant DNA technology, which the following techniques would be most relevant for revealing which of the nucleotides affect metabolism?

Accepted Answer

A)   Homologous recombination
B)   Antisense RNA
C)   RNAi
D)   Artificial synthesis
E)   Mutagenesis

Question 6

Humans have been using biotechnology for _______ years.

Accepted Answer

A)   less than 50
B)   about 100
C)   about 200
D)   about 1,000
E)   at least 8,000

Question 7

A vector designed for the expression of a eukaryotic transgene in E. coli requires

Accepted Answer

A)   a eukaryotic promoter.
B)   a bacterial promoter.
C)   bacterial transcription termination sequences.
D)   bacterial ribosome binding sequences.
E)   b, c and d

Question 8

For which of the following processes would we use homologous recombination?

Accepted Answer

A)   Separating DNA fragments by size
B)   Inactivating specific genes
C)   Examining patterns of expression of genes in different tissues
D)   Identifying specific individuals through their genetic differences
E)   Producing sticky ends

Question 9

Which of the following DNA sequences would most likely be recognized by a restriction enzyme?

Accepted Answer

A)   AGTCGA
B)   GTCTGA
C)   TAAGGT
D)   TAGCTA
E)   TCGTGGA

Question 10

Which of the following best describes the major advantage of using microarrays to detect gene expression differences across the genome, as compared with hybridization technologies?

Accepted Answer

A)   Microarrays are much faster.
B)   Microarrays do not require reverse transcription in order to detect gene expression.
C)   Microarrays do not require the production of synthetic oligonuclotides.
D)   Both a and b
E)   Both b and c

Question 11

Which of the following statements about bacterial antibiotic resistance genes is false?

Accepted Answer

A)   They are usually present in the bacterial large circular genome.
B)   They were used by Cohen and Boyer in their first recombinant DNA experiments.
C)   They are convenient selectable markers.
D)   They can confer antibiotic resistance to other prokaryotes.
E)   They are important to medicine.

Question 12

The primary benefit of using genetic markers in plant breeding is that this technology

Accepted Answer

A)   increases the range of possible new traits that can be introduced into plant breeding.
B)   allows one to perform selective inactivation.
C)   prevents meiosis.
D)   enhances the ability of antisense RNA.
E)   enables more precise breeding because specific desirable genetic variants can be identified and selected.

Question 13

The use of living cells to produce materials that are useful to people is known as _______.

Accepted Answer

The answer of The use of living cells to produce...

Question 14

Use the following to answer questions:
The diagram below shows the steps used in microarray analysis to detect differences in expression between tumor tissue and normal tissue. 
-At which step does hybridization with oligonucleotides occur?

Accepted Answer

A)   Before arrow 1
B)   Between arrows 1 and 2
C)   At arrow 3
D)   At arrow 4
E)   None of the above

Question 15

Which of the following is not an application of recombinant DNA technology?

Accepted Answer

A)   Generating large amounts of tissue plasmonigen factor to help dissolve blood clots
B)   Making plants more resistant to insect larva
C)   Creating vaccines for pathogens
D)   Reducing the salt in environmental soil so that plants can grow
E)   Creating bacteria that can accelerate the breakdown of wood chips and paper

Question 16

For which of the following techniques is the synthesis of oligonucleotides most directly useful?

Accepted Answer

A)   Homologous recombination
B)   Transformation
C)   The polymerase chain reaction
D)   Electroporation
E)   Electrophoresis

Question 17

Use the following to answer questions:
The diagram below shows the steps used in microarray analysis to detect differences in expression between tumor tissue and normal tissue. 
-At which step is antisense RNA used?

Accepted Answer

A)   Before arrow 1
B)   Between arrows 1 and 2
C)   At arrow 3
D)   At arrow 4
E)   None of the above

Question 18

Proteins that are synthesized in the cytoplasm of eukaryotic cells may be targeted to many places within the cell. How might you use rDNA methods to discern where the information is for the targeting of a particular protein?

Accepted Answer

A)   Knock out the gene for the protein
B)   Upregulate the gene for the protein
C)   Express the protein in E. coli
D)   Mutate the gene for the protein, transform the organism, and test the location of the protein in the transformants
E)   Use RNAi technology

Question 19

For a Ti plasmid to be propagated in a host plant cell, the vector needs

Accepted Answer

A)   telomeres.
B)   centromeres.
C)   an origin of replication.
D)   a reporter gene.
E)   a, b, and c

Question 20

Use the following to answer questions :
The diagram below shows how knockout mice are constructed. 
-When does homologous recombination take place?

Accepted Answer

A)   Before arrow 1
B)   Between arrows 1 and 2
C)   Between arrows 2 and 3
D)   Between arrows 3 and 4
E)   After arrow 4

Exam 18: Recombinent Dna and Biotechnology

Use the following to answer questions: The diagram below shows the steps used in microarray analysis to detect differences in expression between tumor tissue and normal tissue. -At which step is antisense RNA used?

Correct AnswerverifiedShow Answer

The use of living cells to produce materials that are useful to people is known as _______.

Correct AnswerverifiedShow Answer

Humans have been using biotechnology for _______ years.

Correct AnswerverifiedShow Answer

The use of which of the following would be most appropriate for inhibiting transcription of a specific gene?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which of the following best describes the major advantage of using microarrays to detect gene expression differences across the genome, as compared with hybridization technologies?

Correct AnswerverifiedShow Answer

Describe three useful products that have been produced using biotechnology. Outline two specific dangers that could result from producing organisms that contain foreign genes.

Correct AnswerverifiedUseful products include rice grains that...View AnswerShow Answer

All other things being equal, which of the following genetically altered foods would most likely be of concern regarding human health?

Correct AnswerverifiedShow Answer

For which of the following processes would we use homologous recombination?

Correct AnswerverifiedShow Answer

Which of the following DNA sequences would most likely be recognized by a restriction enzyme?

Correct AnswerverifiedShow Answer

For which of the following techniques is the synthesis of oligonucleotides most directly useful?

Correct AnswerverifiedShow Answer

The primary benefit of using genetic markers in plant breeding is that this technology

Correct AnswerverifiedShow Answer

Use the following to answer questions: The diagram below shows the steps used in microarray analysis to detect differences in expression between tumor tissue and normal tissue. -At which step does hybridization with oligonucleotides occur?

Correct AnswerverifiedShow Answer

Which of the following statements about bacterial antibiotic resistance genes is false?

Correct AnswerverifiedShow Answer

Use the following to answer questions : The diagram below shows how knockout mice are constructed. -When does homologous recombination take place?

Correct AnswerverifiedShow Answer

A vector designed for the expression of a eukaryotic transgene in E. coli requires

Correct AnswerverifiedShow Answer

Which of the following is not an application of recombinant DNA technology?

Correct AnswerverifiedShow Answer

Proteins that are synthesized in the cytoplasm of eukaryotic cells may be targeted to many places within the cell. How might you use rDNA methods to discern where the information is for the targeting of a particular protein?

Correct AnswerverifiedShow Answer

For a Ti plasmid to be propagated in a host plant cell, the vector needs

Correct AnswerverifiedShow Answer

The process of inserting recombinant DNA is considered transfection when

Correct AnswerverifiedShow Answer

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Useful products include rice grains that...
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