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Exam 25: Dna Replication, Repair and Recombination

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Question 31

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A prokaryotic replisome typically contains two molecules of DNA pol III, but only one molecule of DNA pol I.Why?

A) The DNA pol I works on the leading strand, while DNA pol IIIs work on the Okazaki fragments.since there are several of those, it takes more proteins to keep up.
B) DNA pol I has a built-in proofreading exonuclease; DNA pol III does not.The second DNA pol III is needed to follow the first to accomplish the necessary proofreading.
C) The DNA pol IIIs work at one replication fork, while the DNA pol I works at the other.This way they don't meet exactly at the other side of the chromosome.
D) The DNA pol IIIs do most of the work.DNA pol I only has to work on the telomers.
E) DNA pol I replaces the RNA primers with DNA, which really only needs to be done repetitively on one strand, while both strands are worked on by the DNA pol IIIs.

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Question 32

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When a section of DNA is damaged and polymerization cannot proceed, _____ repair can reconstitute the damaged replication fork.

A) mismatch
B) nucleotide excision
C) recombination
D) photoreactivation
E) base excision

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Question 33

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Which of the following describes the usefulness of primase?

Matching Choose the correct answer from the list. Not all the answers will be used. -G-rich polynucleotides can form _______

Which of the following statements about the fidelity of replication is FALSE?

Lagging stands are polymerized in the ______ direction, and the DNA fragments are about ______ nucleotides (nt) long in eukaryotic cells.

Use the following diagram to complete the sentences below " ______ is the enzyme which catalyzes phosphodiester bond formation on both strands.Processivity of DNA pol is increased when associated with ____.DNA untwisting is accomplished by ______."

Use the following information to answer the next questions : Suppose that an error in replication occurred at position 324 in the E.coli sequence below, and that a GATC motif used to regulate replication and restriction is located about 300 bp away.Note that the A on the bottom (shown by the underlining) strand is methylated. -What enzymes would be used to repair this error?

Suppose Meselson & Stahl had observed the following data in their famous experiment involving a switch from medium containing 15N to 14N.What would they have concluded about the nature of DNA replication?

Use the following information to answer the next questions : Suppose that an error in replication occurred at position 324 in the E.coli sequence below, and that a GATC motif used to regulate replication and restriction is located about 300 bp away.Note that the A on the bottom (shown by the underlining) strand is methylated. -E.coli DNA repair enzymes act on the sequence.Which of the following will result?