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Exam 20: Dna Tools and Biotechnology

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Question 31

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In recombinant DNA methods, the term vector can refer to

A) the enzyme that cuts DNA into restriction fragments.
B) the sticky end of a DNA fragment.
C) a SNP marker.
D) a plasmid used to transfer DNA into a living cell.

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Question 32

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A cell of a particular type, that has the ability to differentiate into other cells of the same tissue type, is a(n) ________ cell.

A) totipotent
B) pluripotent
C) tumour
D) metastatic
E) zygote

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Question 33

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Which of the following techniques used to analyze gene function depends on the specificity of DNA base complementarity?

Sequencing an entire genome, such as that of C. elegans, a nematode, is most important because

-Which enzyme was used to produce the molecule in the figure above?

Genetic engineering is being used by the pharmaceutical industry. Which of the following is not currently one of the uses?

Use the following information to answer the next few questions. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. -Bacteria that do not take up any plasmids would grow on which media?

Which of the following statements is (are) about restriction enzymes? I. They are used by bacteria to cut up foreign DNA. II. They cut DNA unevenly to produce "sticky ends." III. They are non-specific and cut anywhere along a sequence. IV. They are involved with heterochromatin production.

A principal problem with inserting an unmodified mammalian gene into a plasmid, and then getting that gene expressed in bacteria, is that

To introduce a particular piece of DNA into an animal cell, such as that of a mouse, you would find more probable success with which of the following methods?

Use the following information to answer the next few questions. CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes overexpression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early. -One possible use of transgenic plants is in the production of human proteins, such as vaccines. Which of the following is a possible hindrance that must be overcome?

DNA technology has many medical applications. Which of the following is not done routinely at present?

Genetically engineered plants

Use the following figure to answer the questions below. The DNA profiles that follow represent four different individuals. -Which of the following are probably siblings?

Use the following information to answer the questions below. Spider silk is one of the strongest biomolecules known. It has a tensile strength greater than Kevlar and elasticity greater than rubber. Attempts to "farm" spiders have met with little success (they tend to eat each other) . Researchers have identified the gene responsible for the formation of the silk. -Researchers have successfully inserted this gene into goats. These "spidergoats" live a normal life. The silk protein is expressed and extracted from their milk. This is quite controversial; why not manufacture the silk from E.coli?

DNA microarrays have made a huge impact on genomic studies because they

How does a bacterial cell protect its own DNA from restriction enzymes?

One successful form of gene therapy has involved delivery of an allele for the enzyme adenosine deaminase (ADA) to bone marrow cells of a child with SCID, and delivery of these engineered cells back to the bone marrow of the affected child. What is one major reason for the success of this procedure as opposed to many other efforts at gene therapy?

Which of the following would not be true of cDNA produced using human brain tissue as the starting material?

The first cloned cat, called Carbon Copy, was a calico, but she looked significantly different from her female parent. Why?