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Which of the following does not affect microbial nucleic acids?


A) Moist heat
B) Ultraviolet light
C) X rays
D) Ethylene dioxide
E) Formaldehyde

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Hydrogen peroxide is used in the process of sterilizing instruments such as endoscopes.

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The alcohol wipe used on a patient's skin before an injection is an example of ______.


A) antisepsis
B) disinfection
C) sterilization
D) decontamination
E) sanitization

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Removal of moisture by dehydration is called ______.


A) pasteurization
B) flash freeze
C) lyophilization
D) desiccation
E) sterilization

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D

Cold temperatures are considered microbistatic, whereas excessive heat is considered microbicidal. This is due to the fact that _______.


A) in cold temperatures, the energy of activation is not sufficient to drive chemical reactions regardless of the presence of enzymes, whereas excessive heat denatures enzymes
And/or incinerates the cell causing irreparable damage
B) cold temperatures denature enzymes causing irreparable damage to the cell, whereas heat limits the energy of activation available to drive chemical reactions
C) cold temperatures freeze and therefore kill the cell, whereas excess heat keeps the cell metabolically active but not dividing
D) cold temperatures halt binary fission and is therefore considered a sterilant, whereas heat does not kill spores so it is not an effective method of control

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Which of the following represents the use of osmotic pressure as a microbial control method?


A) Bleaching a kitchen counter
B) Salting of meat
C) Rinsing of a cut with Betadine
D) Exposing dental equipment to UV light

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All of the following pertain to hypochlorites except _______.


A) they are found in iodophors
B) they are used to disinfect dairy, restaurant, and medical equipment
C) they are found in common household bleach
D) they release hypochlorous acid in solution
E) they cause denaturation of enzymes

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Alcohols are more effective at inactivating enveloped viruses than naked viruses.

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Surgeons preparing for an invasive surgical procedure will scrub their hands thoroughly, and then they use a strong alcohol-based hand sanitizer which renders their hands sterile.

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Which of the following is not true of glutaraldehyde?


A) It cross-links proteins on cell surfaces.
B) It is classi?ed as a carcinogen.
C) It does not damage plastics.
D) It inactivates viruses.
E) It inactivates endospores in 3 hours.

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The sterilizing gas used in chemiclaves is ______.


A) chlorine dioxide
B) ethylene oxide
C) glutaraldehyde
D) formaldehyde
E) iodophor

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Your friend Joe recently visited the doctor for steroid injections into his knee to reduce swelling due to a previous knee injury. Within a couple of days, he had an infection caused by Pseudomonas, a very pathogenic and drug resistant bacterium. Luckily, Joe went to the doctor immediately and received antibiotics. The doctor tells him that the same problem has occurred to many other people across the United States. Predict the most likely cause of this situation.


A) The bacterium is normally on the skin of people, so can easily access the patient's blood during the needle stick.
B) The needles were re-used and already contaminated before being used on the patients.
C) The staff giving the steroid injections did not use proper aseptic technique.
D) The steroid was contaminated at the production plant, so multiple batches of that drug made at that plant were contaminated with that bacterium.

Correct Answer

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Which of the following acids is not used to destroy or inhibit microbial cells in food?


A) Lactic acid
B) Benzoic acid
C) Phosphoric acid
D) Propionic acid
E) Acetic acid

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Iodophors include ______.


A) alcohols
B) chlorhexidine
C) tincture of iodine
D) chloramines
E) betadine

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Which of the following items are typically irradiated in order to kill microbes?


A) Cured meats
B) Human tissues such as heart valves and skin
C) Operating room air
D) Surgical gloves
E) All of the choices are correct.

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Which of the following methods of microbial control is mismatched?


A) Exposure of surgical equipment to ultraviolet light - radiation
B) Autoclaving nutrient agar before pouring into Petri plates - sterilization
C) Heating a liquid to 71.6oC for 15 seconds - pasteurization
D) Pickling eggs - osmotic pressure

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D

Which of the following microbial forms has the highest resistance to physical and chemical controls?


A) Naked viruses
B) Protozoan cysts
C) Fungal spores
D) Bacterial endospores
E) Yeast

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The removal of all life forms from inanimate objects is termed ______.


A) antisepsis
B) disinfection
C) sterilization
D) decontamination
E) degerming

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Which of the following is true regarding the use of osmotic pressure as an antimicrobial agent?


A) Exposing organisms to the air on a benchtop so they completely dry out is an example of using osmotic pressure as a means of microbial control.
B) Salt is an effective solute to create an osmotic pressure gradient outside of a cell; however, the use of sugar, such as in jams and jellies, works via a different mechanism.
C) Salts and sugars external to a microbe create a hypotonic environment, which causes lysis of the cell.
D) Exposing a microbe to a hypertonic environment draws water out of the cell, causing plasmolysis.

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Scrubbing or immersing the skin in chemicals to reduce the numbers of microbes on the skin is _______.


A) disinfection
B) sterilization
C) sanitization
D) degermation

Correct Answer

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D

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