Question 1

The negative stain is used to

Accepted Answer

A)   determine cell size.
B)   determine Gram reaction.
C)   visualize endospores.
D)   determine flagella arrangement.
E)   visualize capsules.

Question 2

The limit of resolution of the compound microscope illuminated with visible light is approximately
0.2 μm.

Accepted Answer

A) True 
 B)False

Question 3

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Accepted Answer

A)   brightfield microscope
B)   phase-contrast microscope
C)   scanning electron microscope
D)   darkfield microscope
E)   transmission electron microscope

Question 4

Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?

Accepted Answer

A)   darkfield microscope
B)   electron microscope
C)   phase-contrast microscope
D)   compound light microscope
E)   fluorescence microscope

Question 5

Both phase-contrast microscopy and differential interference contrast microscopy are used to view the internal structures of cells without staining.

Accepted Answer

A) True 
 B)False

Question 6

The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.

Accepted Answer

A) True 
 B)False

Question 7

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Accepted Answer

A) True 
 B)False

Question 8

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

Accepted Answer

A)   capsules.
B)   flagella.
C)   endospores.
D)   cell walls.
E)   The answer cannot be determined.

Question 9

Which microscope is used to see intracellular detail in a living cell?

Accepted Answer

A)   fluorescence microscope
B)   transmission electron microscope
C)   brightfield microscope
D)   atomic force microscope
E)   two-photon microscope

Question 10

Which microscope uses two beams of light to produce a three-dimensional color image?

Accepted Answer

A)   phase-contrast microscope
B)   DIC microscope
C)   electron microscope
D)   fluorescence microscope
E)   darkfield microscope

Question 11

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?

Accepted Answer

A)   purple
B)   red
C)   colorless
D)   brown

Question 12

Which microscope can be used to visualize DNA or botulinum toxin?

Accepted Answer

A)   compound light microscope
B)   phase-contrast microscope
C)   scanning tunneling microscope
D)   scanning electron microscope
E)   confocal microscope

Question 13

What structure does light pass through after leaving the condenser in a compound light microscope?

Accepted Answer

A)   illuminator
B)   objective lens
C)   specimen
D)   ocular lens

Question 14

Which of the following pairs is mismatched?

Accepted Answer

A)   confocal microscope ‐ produces a three-dimensional image
B)   scanning electron microscope ‐ produces a three-dimensional image
C)   fluorescence microscope ‐ uses a fluorescent light
D)   darkfield microscope ‐ uses visible light
E)   scanning tunneling microscope ‐ allows visualization of atoms

Question 15

Which microscope is used to see internal structures of cells in a natural state?

Accepted Answer

A)   fluorescence microscope
B)   phase-contrast microscope
C)   compound light microscope
D)   electron microscope
E)   darkfield microscope

Question 16

Which of the following pairs is mismatched?

Accepted Answer

A)   methylene blue ‐ simple stain
B)   crystal violet ‐ simple stain
C)   nigrosin ‐ negative stain
D)   basic dye ‐ negative stain
E)   acidic dye ‐ capsule stain

Question 17

Which microscope is best used for observing the surfaces of intact cells and viruses?

Accepted Answer

A)   darkfield microscope
B)   phase-contrast microscope
C)   scanning electron microscope
D)   brightfield microscope
E)   fluorescence microscope

Question 18

Which microscope is used to observe viruses and the internal structure of thinly sectioned cells?

Accepted Answer

A)   brightfield microscope
B)   scanning electron microscope
C)   darkfield microscope
D)   fluorescence microscope
E)   transmission electron microscope

Question 19

A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear

Accepted Answer

A)   smaller than it would if immersion oil was used.
B)   somewhat fuzzy and have poor resolution.
C)   the same as it would if the immersion oil was used.
D)   larger than it would if immersion oil was used.
E)   to have no color.

Question 20

Which of the following places the steps in the correct sequence? 1- Staining
2- Making a smear 3-Fixing

Accepted Answer

A)   3-2-1
B)   1-2-3
C)   1-3-2
D)   2-3-1
E)   The order is unimportant.

Exam 3: Observing Microorganisms Through a Microscope

Which microscope can be used to visualize DNA or botulinum toxin?

Correct AnswerverifiedShow Answer

Which of the following pairs is mismatched?

Correct AnswerverifiedShow Answer

The greater resolution of the electron microscope compared to the compound microscope is due to the longer wavelengths of the electrons used to examine specimens.

Correct AnswerverifiedShow Answer

Which microscope is used to see intracellular detail in a living cell?

Correct AnswerverifiedShow Answer

Scanned probe microscopy is used to examine fine detail of molecular complexes, such as blood clots, or molecules, such as DNA.

Correct AnswerverifiedShow Answer

Which microscope is used to observe viruses and the internal structure of thinly sectioned cells?

Correct AnswerverifiedShow Answer

Which microscope uses two beams of light to produce a three-dimensional color image?

Correct AnswerverifiedShow Answer

Both phase-contrast microscopy and differential interference contrast microscopy are used to view the internal structures of cells without staining.

Correct AnswerverifiedShow Answer

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

Correct AnswerverifiedShow Answer

The limit of resolution of the compound microscope illuminated with visible light is approximately 0.2 μm.

Correct AnswerverifiedShow Answer

Which of the following places the steps in the correct sequence? 1- Staining 2- Making a smear 3-Fixing

Correct AnswerverifiedShow Answer

What structure does light pass through after leaving the condenser in a compound light microscope?

Correct AnswerverifiedShow Answer

Which of the following pairs is mismatched?

Correct AnswerverifiedShow Answer

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the greatest magnification you can use to see this structure?

Correct AnswerverifiedShow Answer

Which microscope is best used for observing the surfaces of intact cells and viruses?

Correct AnswerverifiedShow Answer

The negative stain is used to

Correct AnswerverifiedShow Answer

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?

Correct AnswerverifiedShow Answer

Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?

Correct AnswerverifiedShow Answer

Which microscope is used to see internal structures of cells in a natural state?

Correct AnswerverifiedShow Answer

A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear

Correct AnswerverifiedShow Answer

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