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Organisms that can grow with or without oxygen present are


A) aerotolerant anaerobes.
B) obligate anaerobes.
C) facultative anaerobes.
D) obligate aerobes.
E) either facultative anaerobes or aerotolerant anaerobes.

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A clinical sample of (urine/sputum/tissue) is called a biopsy.

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Obligate anaerobes may be cultured in the laboratory


A) in standard complex media.
B) in standard Petri plates.
C) in a standard incubator.
D) in a reducing medium.
E) on blood agar plates.

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Reactions catalyzed by superoxide dismutases can produce the (hydroxyl/peroxide/superoxide) radical.

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Bacteria living in communities of microbes change the genes they are expressing in response to


A) decreasing oxygen.
B) changes in local temperature.
C) changes in hydrostatic pressure.
D) quorum-sensing molecules.
E) changes in pH.

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The conversion of nitrogen gas to ammonia is called nitrogen (metabolism/fixation).

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Which of the following quantification techniques can distinguish living cells from dead cells in a culture?


A) microscopic counts
B) a Coulter counter
C) turbidity
D) dry weight
E) metabolic activity

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A microorganism found living under conditions of high is a barophile.


A) salt concentrations
B) oxygen concentrations
C) hydrostatic pressure
D) carbon dioxide levels
E) pH values

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An aquatic microbe that can grow only near the surface of the water is probably which of the following?


A) a chemotroph
B) a lithotroph
C) a heterotroph
D) a phototroph
E) an anaerobe

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Thermoduric mesophiles are often responsible for spoilage of improperly canned foods.

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An obligate anaerobe can be cultured in a candle jar.

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Agar is a useful compound in the microbiology lab because it is an excellent nutrient for bacteria.

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At temperatures higher than the maximum growth temperature for an organism,


A) proteins are permanently denatured.
B) hydrogen bonds are broken and proteins are permanently denatured.
C) membranes become too fluid for proper function.
D) hydrogen bonds are broken, proteins are denatured, and membranes become too fluid.
E) hydrogen bonds within molecules are broken.

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The generation time of a bacterial culture is determined by the culture conditions.

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A microbe that grows only at the bottom of a tube of thioglycollate medium is probably a(n)


A) obligate anaerobe.
B) microaerophile.
C) obligate aerobe.
D) aerotolerant anaerobe.
E) facultative anaerobe.

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You open a jar that has been in the refrigerator for a couple of weeks and find the contents do not look or smell right. What type of microbe has been growing in your refrigerator?


A) hyperthermophile
B) thermoduric
C) thermophile
D) mesophile
E) psychrophile

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An epidemiologist is investigating a new disease and observes what appear to be bacteria inside tissue cells in clinical samples from victims. The scientist wants to try to isolate the bacteria in the lab. What culture conditions are most likely to be successful?


A) culturing on blood agar plates
B) incubation in an anaerobic culture system
C) inoculation of EMB plates
D) inoculation of a minimal medium broth
E) inoculation of cell cultures

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Halophiles are found living in high conditions.


A) salt concentration
B) oxygen levels
C) pH
D) hydrostatic pressure
E) carbon dioxide

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Biofilms are the natural state for the majority of bacteria in nature.

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Which of the following explains why honey can be stored at room temperature for years without spoiling?


A) hydrostatic pressure
B) osmotic pressure
C) low pH
D) lyophilization
E) anaerobic conditions

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